Total flavonoids from Lagerstroemia speciosa (L.) Pers inhibits TNF-α-induced insulin resistance and inflammatory response in 3T3-L1 adipocytes via MAPK and NF-кB signaling pathways

Adipocyte tissue inflammation orchestrates the occurrence and development of diseases induced by obesity. Here, effects Lagerstroemia speciosa (L.) Pers total flavonoids (LTF) on TNF-α-induced insulin resistance (IR) inflammatory response in 3T3-L1 adipocytes underlying mechanisms were studied. Cell glucose uptake test confirmed that TNF-α could induce IR adipocytes. The sensitivity to was increased LTF treatment. phosphorylation IRS1 Akt TNF-α-treated markedly restored results revealed interleukin-6 (IL-6) monocyte chemoattractant protein-1 (MCP-1) mRNA expression elevated TNF-α-administered adipocytes, whereas adiponectin (ADPN) considerably decreased. When treated with 50 μg/mL LTF, IL-6 MCP-1 levels drastically reduced, ADPN level significantly raised. Moreover, concentration supernatant medium decreased after treatment compared model group. Western blotting showed p38, JNK, ERK mitogen-activated protein kinase (MAPK) signaling pathways IкB NF-кB pathway inhibited. It suggested improves via inhibiting MAPK pathways.